发布日期：2024-02-04 17:11 浏览次数：
| 名称： | anti- Vimentin antibody |
|---|---|
| 货号： | FNab09409 |
| 适应种属： | 人源,大鼠,小鼠 |
| 规格： | 100ug(微克) |
| 状态： | 液体 |
| 纯化方法： | 免疫亲和纯化 |
| 纯度： | 经SDS-PAGE分析,纯度大于95% |
| 宿主： | 兔 |
| 亚型： | lgG |
| 存储： | 用添加了0.02%叠氮化钠和50%甘油的PBS溶解,PH值7.3,可在-20℃下保存12个月（避免反复冻融） |
| 免疫原： | vimentin |
| 免疫原别称： | FLJ36605 antibody, VIM antibody, Vimentin antibody |
| 分子量： | 55 kDa |
| 验证应用： | ELISA, WB, IHC, IF |
| 建议稀释比例： | WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200 |
| UniProt ID： | P08670 |
| 克隆性： | 多克隆 |
| 背景介绍： | Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha(TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10(124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. |
| 图像： |  Immunofluorescent analysis of HepG2 cells, using FNab09409(VIM antibody) at 1:25 dilution。  Immunohistochemistry of paraffin-embedded human ovary cancer using FNab09409(VIM antibody) at dilution of 1:100。  Hela cells were subjected to SDS PAGE followed by western blot with FNab09409(VIM antibody) at dilution of 1:1000。 |